Can you analyse a whole blood sample using a western blot (primary IgG custom antibody, and a secondary anti-IgG horseradish peroxidase)?
I am under the impression that the heme in blood some how interferes with the peroxidase.
But I can not find anywhere any reason why!?
Any help would be very much appreciated
Edited by Chazzer90, 24 March 2014 - 07:09 AM.