I did gram staining of a 24h old B. megaterium culture from plate. Some bacteria stain positive, some negative. I read that endospore forming organisms are prone to staining gram negative if they are not fresh. But my culture was fresh. What happened?
Then, I did another Gram Stain of bacteria I isolated from animals.On Columbia sheep blood agar and LB agar they are growing in small yellow colonies (after 24h incubation). For me it looks like M. luteus. What do you think? Are there other species these bacteria could be?
Thanks for your Help.