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2 Photos - 2 Questions: Gram Stain


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#1 tretol

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Posted 23 March 2014 - 02:05 PM

Hello,

 

I did gram staining of a 24h old B. megaterium culture from plate. Some bacteria stain positive, some negative. I read that endospore forming organisms are prone to staining gram negative if they are not fresh. But my culture was fresh. What happened?

 

Then, I did another Gram Stain of bacteria I isolated from animals.On Columbia sheep blood agar and LB agar they are growing in small yellow colonies (after 24h incubation). For me it looks like M. luteus. What do you think? Are there other species these bacteria could be?

 

 

http://tinyurl.com/ptacx97

 

 

Thanks for your Help.



#2 bob1

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Posted 24 March 2014 - 11:45 AM

It could be that you didn't do your Gram stain properly...

 

It could be Micrococcus luteus, but there are other Gram +ve cocci that grow as yellow colonies... where did you get the sample?  scale is important in images!



#3 Phil Geis

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Posted 25 March 2014 - 03:44 AM

Micrococcus spp. are oxidase positive - give it a try.



#4 tretol

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Posted 02 April 2014 - 10:02 PM

Hi I did oxidase test which was negative. now I'll sequence 16s and see what it is thanks for the help!

#5 tretol

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Posted 07 April 2014 - 11:51 AM

It is M luteus although it proved negative for oxidase!




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