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Reference genes in miRNAs

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#1 Mohamed 1984

Mohamed 1984


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Posted 22 March 2014 - 06:44 AM

Dear All, 


I read many papers about miRNA. In the real time PCR for miRNA detection, I used the miRNA ct values in the ctrl wells as a standard gene. Is this true that in miRNA qPCRs, u do not need a standard gene like in mRNA ? 

#2 pcrman



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Posted 09 April 2014 - 03:16 PM

No, what you described is not correct. You need to have at least one internal control such as sn/snoRNAs. Please read this.

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