Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Reference genes in miRNAs


  • Please log in to reply
3 replies to this topic

#1 Mohamed 1984

Mohamed 1984

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 80 posts
4
Neutral

Posted 22 March 2014 - 06:44 AM

Dear All, 

 

I read many papers about miRNA. In the real time PCR for miRNA detection, I used the miRNA ct values in the ctrl wells as a standard gene. Is this true that in miRNA qPCRs, u do not need a standard gene like in mRNA ? 



#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 09 April 2014 - 03:16 PM

No, what you described is not correct. You need to have at least one internal control such as sn/snoRNAs. Please read this.



#3 medibio

medibio

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 06 June 2014 - 12:29 PM

Yes, you will need at least one endogenous control for normalization, but if you use multiple controls, it will be better.



#4 sialic acid

sialic acid

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 01 July 2014 - 05:17 AM

Question, why can't you use GAPDH/B-actin for endogenous controls like you do a lot of time for qrtPCR for miRNA pcr?  






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.