I am bit confused about the use of dUTP and UDG (uracil glycosylase) for RT-PCR,
I know that there is a protocol to contain dUTP with dNTP for PCR and treat with UDG to prevent the carry over contamination, and I do understand the mechanism about how UDG works.
But my question is I still do not understand the importance of this methods. where the carry over comes and how it affects the result.
Does anyone give me good practical example for this??? or Am I missing something?????
Thanks in advance