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mRNA copies of each gene under different promoters


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#1 kate-hamster

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Posted 20 March 2014 - 12:52 PM

Hi;

how may copies of mRNA do you get from each plasmid in mammalian cells, transient transfection, under different promoters?

 

I am looking for some comparison, eg. how many copies of mRNA from each gene under CMV versus CAG or SV40 or whatever else.

I found this paper http://www.plosbiolo...al.pbio.0040309 that seems to suggest that number of copies of mRNA is around <50, but I dont know what to think about it.
 
Thanks for any references!

 



#2 bob1

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Posted 23 March 2014 - 12:25 AM

This is one of those things that is virtually impossible to measure, as it is very hard to determine how many copies of the plasmid are present in a cell, though in theory this can be measured by qPCR.  So - short answer: you aren't likely to find this information.






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