I forgot to do a heat shock when transforming e.coli. I just had the cells on ice (after adding competent cells to the plasmids) for 15 minutes and then I had to do the heat shock followed by adding the LB medium. However I forgot to do the heatshock.
Now I wonder: has anyone done this before? Do you still have growth?
I am going to simple use the cells anyway, to see if it will still work.. (and I cant redo it anyway at the moment), but just curious if anyone had a similar problem.
Theoretically one might say it could still work.. but curious you ever had a similar problem.