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FISH probe labeling - size too long

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#1 biofingers



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Posted 17 March 2014 - 02:45 PM

Hi, Everyone,

I was using biotin or DIG nick translation mix (Roche) to label FISH probes, but could not get the expected size (200-500bp) even after 5 hours of 16degree incubation. The protocol suggested 2-3 hours. And the kit is NOT expired. Anyone had the same problem? 



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