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Any idea of the composition of the RNA Wash Buffer from Zymo Research RNA Clean


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#1 marek82313

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Posted 17 March 2014 - 12:57 PM

Dear all,

 

does anybody has any idea concerning the composition of the RNA Wash Buffer from Zymo Research RNA Clean & Concentrator kit? It contains EtOH (80%), so I guess whether I can simply use 80% EtOH as "backup" when the buffer is almost finished.

 

Thanks,

 

all the best,

 

Mark



#2 phage434

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Posted 17 March 2014 - 02:44 PM

A guess -- it would be buffered at pH 7.5 (or perhaps lower -- you could measure it) likely with Tris. Make sure it is RNAse free.






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