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How to calculate MOI?

calculation virus MOI

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#1 M.B.T

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Posted 17 March 2014 - 05:55 AM

Hi every one,

 

I need to design an experiment and I need to Transduce my cells with vector dilution MOI 50.

Number of my cell is 5x10e5

Virus titter is 10e8

I am not sure how to calculate MOI, could you please help me with this one.

 

 

Cheers

 

 



#2 bob1

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Posted 17 March 2014 - 11:55 AM

MOI is multiplicities of infection - a moi of 1 would mean that every cell receives 1 infectious unit, a moi of 50 is 50 x that number...

 

5 x 10^5 *50 =??? (this is n in the following equation)

 

then use v=n/c to get the volume of your virus that you require.







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