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Transwell experiments - plating cells on the bottom


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#1 Smarastoni

Smarastoni

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Posted 16 March 2014 - 08:04 PM

Hi, i am trying to plate cells on the bottom of 8micron transwell for migration assays. I have no problems in obtaining a monolayer on the top of the transwell while it is more difficult to obtain it on the bottom. Is there anyone who have experience with this kind of experiments who can help me , please.
Thanks




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