I am culturing the fibroblast cells. My cells become unhealthy every a few generations. But my colleagues are OK. The only differences are the incubation and the FBS and Pen strep concentration. I put exactly 10% FBS and 1% Pen Strep. They just put 50 mL and 5mL respectively to the medium. For me, I put 56.1mL and 5.61mL. Why do I ask here because I am wondering the affection of a little higher FBS and Pen Strep is so much! I paid attention all of the tricky stuffs for cell culture. Somebody can give me some suggestions or something else?
Of course, I will try to low down the FBS and Pen Strep concentration as my colleague is doing to see what happens.