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help with total RNA isolation

multiple bands second band more strong

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#1 raydurga

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Posted 12 March 2014 - 08:18 PM

Hii friends, 

I am doing total RNA isolation from Corynebacterium Glutamicum, my problem is, after running TAE RNA agrose gel,  I found 3 sharp bands on the gel.

 I am attaching a pic of the gel... Sorry it was my first time so I loaded a bit too much sample in the gel...

I am using RiboPure™-Bacteria Kit and the samples r DNAse treated.    please suggest..

 

 

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  • RNA.jpg


#2 phage434

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Posted 13 March 2014 - 10:34 AM

So, what is the problem? These look (mostly) like typical RNA samples, with a 16S, 23S, and 5S/tRNA band.







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