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Anti-Guinea Pig IFN-g and TNF-a - Antibody Suggestions?!


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#1 ctingey

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Posted 12 March 2014 - 12:23 PM

Hello everyone,

 

I am having trouble finding flow antibodies to look at IFN-gamma and TNF-alpha in guinea pig samples. So far I've found that Novus has an ab for TNF-alpha with cross-reactivity in guinea pigs, but that's pretty much it. I was wondering if anyone had prior experience or suggestions. I'm not picky about the fluorophore these antibodies are conjugated to.

 

Thanks!

-Colleen

 



#2 bob1

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Posted 12 March 2014 - 02:46 PM

They are distantly related to rats and mice, so I would try antibodies against those species.  The major problem is that guinea pigs aren't all that common as a lab animal for these sorts of studies (more used for physiology/anatomy and sociology), so finding antibodies that have been tested will be difficult.



#3 MeiYee

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Posted 13 March 2014 - 03:14 AM

Hi Colleen,

 

Yes, it's difficult to find an AB that has been tested for flow cytometry.

 

We offer a free trial size antibody for evaluation if you are interested in testing one of our TNF-alpha antibodies in FC.

 

Please visit St John's Laboratory website  http://www.stjohnsla...Partners-Scheme

 

We only have 1 IFN gamma antibody, but a lot more TNF alpha. If you are outside the UK, we will need to charge shipping

 

Hope this helps

 

Mei 






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