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Can shRNA be picked up by sequencing?


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#1 kaveh

kaveh

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Posted 10 March 2014 - 02:28 PM

Imagine you are doing a shRNA experiment. You knock down the gene of interest by shRNA and you study the transcriptome. If part of a shRNA is complementary to the target mRNA, once the next-gen sequencing library is being made, doesn't shRNA become converted to cDNA? Wouldn't this be sequenced and read as the target RNA in sequencing? 

 

Thanks. 

 

KD






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