Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Need help to identify bands in AGE result

is this smearing?

  • Please log in to reply
3 replies to this topic

#1 fam

fam

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 10 March 2014 - 06:02 AM

i couldn't identify whether it is having smearing or bands,according to some of my laboratory seniors,some said is bands while some said it just having smearing result...10 march modify.jpg



#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 10 March 2014 - 07:47 AM

These look like very short fragments, probably primer-dimers. It's not a specific product, unless it is perhaps a very small fragment.



#3 fam

fam

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 10 March 2014 - 10:13 PM

The product size is compatible with the DNA ladder which around 227-272bp,and when I checked on NCBI primer BLAST,it targeted to specific primers: PIN1and GAPDH ,perhaps still got other factors to cause me get the failed result?

#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 11 March 2014 - 05:01 AM

If you expect short fragments, you should use a lower percentage gel and run the gel longer to get better resolution.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.