Hi, I am meeting problems with my IP. I have a protein and, I get 3 fragment of this protein expressed, and trying to see which one is responsible for the binding.
However, while the C-terminal and N-terminal fragment is Okay to express, I can't see any signal for the middle fragment, I think it was because the protein expressed was degraded in vivo before I extract my protein.
So will it be any better if I collect my cells earlier like 24 hrs rather than 48 hrs post-transfection??
Anyone ever experience this? Thanks!!!