Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

MTT assay without positive control


  • Please log in to reply
1 reply to this topic

#1 elise



  • Members
  • Pip
  • 1 posts

Posted 08 March 2014 - 06:58 PM

Hello. i wanna ask is MTT assay valid if there is no positive control included for cytotoxicity study?


In my assay, i only include


MTT control = no cell + mtt + dmso

SOLVENT control = cell + medium with solvent used to dissolved the compound (6 half serial dilution starting with 0.1% conc. of the solvent) + mtt + dmso

TEST = cell + medium with compound (6 half serial dilution) + mtt + dmso

UNTREATED control = cell + medium only + mtt + dmso


is my assay valid? and is the solvent control in my assay considered to be negative control? and what actually is the function of positive control?



#2 vinclam



  • Members
  • Pip
  • 2 posts

Posted 20 August 2014 - 05:11 AM

The 'untreated control' should be your positive control, isn't it?

positive control is used as a reference for your sample cell viability, it is always been set as 100% cell viability.

I used the following formula to calculate cell viability:

Cell viability (%) = (OD of sample / OD of positive control) x 100


Your MTT assay is valid and the 'MTT control' should be your negative control as it supposedly wont give any result as there's no cell.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.