Hello. i wanna ask is MTT assay valid if there is no positive control included for cytotoxicity study?
In my assay, i only include
MTT control = no cell + mtt + dmso
SOLVENT control = cell + medium with solvent used to dissolved the compound (6 half serial dilution starting with 0.1% conc. of the solvent) + mtt + dmso
TEST = cell + medium with compound (6 half serial dilution) + mtt + dmso
UNTREATED control = cell + medium only + mtt + dmso
is my assay valid? and is the solvent control in my assay considered to be negative control? and what actually is the function of positive control?
Answered
