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MTT assay without positive control

MTT ASSAY POSITIVE CONTROL

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#1 elise

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Posted 08 March 2014 - 06:58 PM

Hello. i wanna ask is MTT assay valid if there is no positive control included for cytotoxicity study?

 

In my assay, i only include

 

MTT control = no cell + mtt + dmso

SOLVENT control = cell + medium with solvent used to dissolved the compound (6 half serial dilution starting with 0.1% conc. of the solvent) + mtt + dmso

TEST = cell + medium with compound (6 half serial dilution) + mtt + dmso

UNTREATED control = cell + medium only + mtt + dmso

 

is my assay valid? and is the solvent control in my assay considered to be negative control? and what actually is the function of positive control?

 

 



#2 vinclam

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Posted 20 August 2014 - 05:11 AM

The 'untreated control' should be your positive control, isn't it?

positive control is used as a reference for your sample cell viability, it is always been set as 100% cell viability.

I used the following formula to calculate cell viability:

Cell viability (%) = (OD of sample / OD of positive control) x 100

 

Your MTT assay is valid and the 'MTT control' should be your negative control as it supposedly wont give any result as there's no cell.







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