Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

PE and FITC


  • Please log in to reply
1 reply to this topic

#1 kundanitesh

kundanitesh

    member

  • Members
  • Pip
  • 1 posts
1
Neutral

Posted 07 March 2014 - 02:22 AM

Hi,

 

I am new to using FACS and now I am confused if I can use FITC and PE together as a muticolor application? I read somewhere that these channels have spillover into each others signals and might give misreadings. Could someone help me answer this question.

 

Thanks.



#2 Tabaluga

Tabaluga

    Making glass out of shards

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 362 posts
47
Excellent

Posted 07 March 2014 - 03:14 AM

This is correct, PE and FITC can spill over and you need to do compensation. Which FACS machine/software are you working with ?
As an alternative I could recommend you using FITC and Alexa647, if you can choose your colours, they usually work without compensation

Edited by Tabaluga, 07 March 2014 - 03:15 AM.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.