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questions on transwell assay


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#1 Evaaaa

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Posted 06 March 2014 - 11:47 PM

Hi, I am facing the problems when I do transwell assay to detect cell migration. But the cells looks very "ugly" when i take pictures. Does anyone know how to keep the cell pretty? I wash the transwell with PBS, did I wash it too much? 



#2 bob1

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Posted 07 March 2014 - 11:39 PM

Can you add a pic of these "ugly" cells?  At which point are they "ugly" - before or after migration?



#3 Evaaaa

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Posted 08 March 2014 - 12:45 AM

Can you add a pic of these "ugly" cells?  At which point are they "ugly" - before or after migration?

After migration. They look "broken" 



#4 bob1

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Posted 08 March 2014 - 12:16 PM

What sort of broken?  apoptotic? senescent? ragged? vacuolated? change of shape?

 

What cells are these?  Is is possible that they are differentiating?






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