I have been working on repeating an EMSA shift from the lit. I am using DIG gel shift kit vers 2 from Roche.
I have compl. synthetic ssDNA oligos 30bp. Anneal myself. then label while labeling a pre-annealed ctrl.
After running my gels on novex precast 6% DNA retard. gels I get a nonspecific band in my free probe only lane as well as my labeled probe with pure proteins but not in my controls. See image please.
What is this non-spec band how do I get rid of it?