Recently I've been staining my mouse hearts with H&E. Although the staining works, my nuclei seems too faint, and my eosin is extremely pink. I've tried decreasing the time for the Eosin from 4 minutes to 2 minutes, and increasing the time for Hematoxylin from 5 minutes to 7 minutes, but the results are the same. I am using Mayer's H&E from Fisher to stain these slides. The fixation time is one day in formaldehyde, and the sections are 6uM thick. Any experts out there have any idea?
Thanks a bunch!