I'm a bit confused regarding Gibson assembly or SLIC. Using an exonuclease should result in regression of both 5' ends, right? Why is in all schemes only one of them degraded ( https://www.neb.com/...bly-cloning-kit )? Isn't there a chance that the whole PCR product will be eventually degraded? Or are they using modified primers?
Edited by Rsm, 05 March 2014 - 05:55 AM.