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Low RNA quantity after on column DNAse digestion


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#1 Mohamed 1984

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Posted 04 March 2014 - 01:15 PM

Dear All, 

 

I am faced something strange. I tried two time with different DNAse digestion which I am sure is RNAse free, to remove the genomic DNA from the RNA samples just prior to a deep sequencing experiment. 

 

Is there are any other reasons that the RNA is reduced in quantity after on column digestion ?

 

please suggestions to correct 



#2 perneseblue

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Posted 04 March 2014 - 08:16 PM

RNAse contamination from your pipette or water?


May your PCR products be long, your protocols short and your boss on holiday




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