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Conjugation of Ab to magnetic beads to isolate T cells from Spleen

T cell Isolation MACS

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2 replies to this topic

#1 Monsoon_boy

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Posted 04 March 2014 - 01:26 AM

Hi there,

 

I want to isolate CD8 T cells from spleenocytes, I have both magnetic beads and a normal anti CD 8 antibody with me. Is there a way I can conjugate this Ab to the beads to get a pure population. After conjugation can I follow the normal procedures laid down by MACS for seprating cells?

 

Thanks in advance.

 

 

MB



#2 Missle

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Posted 04 March 2014 - 08:26 AM

are the magnetic beads functionalized?



#3 Eve Technologies

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Posted 29 May 2014 - 10:33 AM

Your magnetic beads need to be either carboxylated or precoated with avidin (which requires a biotinylated antibody).

 

 

Here is a guide for coupling protein to microspheres / beads:

 

http://info2.luminex...e-xmap-cookbook

 

Luminex provides magnetic beads that are funtionalized.






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