I wonder if it is the right subforum to ask, but since it has something to do with PCR product, I'm gonna ask here.
I purified my PCR product (excised the single band, and using column from roche kit to purify it) and I want to use this product as standard for the absolute quantification real-time PCR project. Thing is, I have to know the exact concentration of this purified PCR product, so that I can convert the concentration (ng/ul) to copy molecules/ul using Avogadro equation. From the journal, the researchers use picogreen to quantify that purified PCR product, but since I don't have such the equipment nor the reagen, I wonder if it is okay to quantify the purification product's concentration with Nanodrop?
I really hope you guys can help me with my question.