Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

SDS PAGE sample prep

sds page sample prep calculations

  • Please log in to reply
3 replies to this topic

#1 CENSIS

CENSIS

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 March 2014 - 02:42 PM

Hello:

 

I need help figuring out the calculations for this sample prep:

 

Prepare samples to a final conc. of 0.2 micrograms/microliter with sample buffer (25% v/v), reducing agent (10% v/v) and water. For samples of different conc. adjust the dilutions proportionally.

 

Nominal sample conc.               sample                sample buffer          reducing agent        water

(% w/w)                                       (ul)                           (ul)                           (ul)                      (ul)

 

0.3                                                 2                            7.5                             3                       17.5

 

0.2                                                 3                             7.5                            3                       16.5

 

0.1                                                 6                             7.5                             3                       19.5

 

My samples are in liquid.



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,726 posts
399
Excellent

Posted 03 March 2014 - 03:24 PM

So what are the concentrations of your samples.... the table I see here doesn't seem to have any obvious problems.



#3 CENSIS

CENSIS

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 05 March 2014 - 04:33 PM

The sample concentrations are in the first column, as (% w/w).

How can you start with a 0.2% w/w solution, add 3ul of this to the

total volume of 30ul, and end up with 0.2ug/ul ? I'm confused!



#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,726 posts
399
Excellent

Posted 06 March 2014 - 12:10 AM

0.2% would be 2 mg/ml ((2/1000) *100) or 2 ug/ul, diluted 1:10 (or 3 in 30) would give you 0.2 ug/ul. 







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.