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RT-qPCR - delta delta Ct with undetermined expression


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#1 Aravin

Aravin

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Posted 03 March 2014 - 01:33 PM

Hello,

 

I am comparing the effects of a specific treatment on a gene. In my control samples, the gene is undetected, so very low copies I presume, and I am getting Cts of about 26-30 in the treatment group. 

 

I can't do delta delta Ct with this method, nor do I know how to display this data effectively in a graph. What is the best way to compare the expression between control and Tx group? Also, how would you depict the results in a graph? 

 

Thanks a lot!

 






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