I am comparing the effects of a specific treatment on a gene. In my control samples, the gene is undetected, so very low copies I presume, and I am getting Cts of about 26-30 in the treatment group.
I can't do delta delta Ct with this method, nor do I know how to display this data effectively in a graph. What is the best way to compare the expression between control and Tx group? Also, how would you depict the results in a graph?
Thanks a lot!