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Autoclaving microcentrifuge / eppendorf tubes

eppendorf tubes autoclave

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6 replies to this topic

#1 p.phadtare

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Posted 03 March 2014 - 08:26 AM

Hi,

 

Please let me know the the correct way of autoclaving eppendorf tubes. :)



#2 jerryshelly1

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Posted 03 March 2014 - 08:50 AM

We usually put the tubes in a Nalgene container able to withstand higher temperatures (121C). This container can be an open top that you can place aluminum foil over or it can be a sealable container. To verify that the autoclave reached the appropriate temperature, we use heat/pressure sensitive tape. The cycling conditions vary, but we usually do 15-30 minutes depending upon the volume.



#3 phage434

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Posted 03 March 2014 - 03:57 PM

Or buy them sterile. Autoclaves add a lot of 'extras' to your samples.



#4 Ameya P

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Posted 06 March 2014 - 10:07 PM

A lot of companies offer sterile tubes now a days. Some even give you tubes that are DNase RNase free for setting up your PCRs


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#5 p.phadtare

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Posted 11 March 2014 - 08:24 AM

Thanks a lot everyone! But I ended up autoclaving them simply in large glass beaker for 20 minutes (since our lab doesn't have the Nalgene Containers). :)



#6 Ameya P

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Posted 12 March 2014 - 01:20 AM

Just make sure you dry them sufficiently before using


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#7 p.phadtare

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Posted 14 March 2014 - 10:00 AM

Sure, thanks! :)







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