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How to control the pH of water after sterilization ?

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3 replies to this topic

#1 Nagarjuna



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Posted 28 February 2014 - 08:43 AM

Dear Forum members,


I want to formulate a parenteral preparation whose pH should be between 10.5 to 11.10;

Here medium is water, the pH of the water adjusted to 11.00 with 0.1N NOH solution, after sterilization the pH decresed to 7.5, why so ?

Without teriziation pH of water is decreasing to 10.5.

Is any alternate is there to maintain the pH (even after sterilization) ?

#2 hobglobin


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Posted 28 February 2014 - 10:03 AM

One possible reason is that dissolved carbon dioxide decreases pH:
CO2 + H2O <--> H2CO and then H2CO3 + H2O <--> HCO3- + H3O+


Carbonic acid is a weak acid but might be enough to decrease pH in your unbuffered system. ITo avoid this use a buffer, actually for this alkaline pH, I'm not sure which one.

Another possibility would be pH adjustment after autoclaving (if you did this, you just wrote sterilisation) and then avoid contact to air.

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#3 perneseblue


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Posted 28 February 2014 - 06:19 PM

Given that an autoclave system is also pressurized hobglobin suggestion is quite likely correct. The high pressure would drive the carbon dioxide to dissolve into yourbuffer, and you will obtain a sodium hydrogen carbonate solution.


I would suggest that you use a buffer system and filter sterilize your solution. 


As for what buffer to you, I am not sure what you will be using the solution for, but there are several options



The table above gives CABS (4-(Cyclohexylamino)-1-butanesulfonic acid ) as an option.


You could also use an amino acid as a buffer.



Lysine, Proline, Cysteine and Tyrosine are within the 10.5 to 11 range.

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#4 Curtis


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Posted 01 March 2014 - 11:06 AM

I've seen many people autoclave their bottles with loose cap, without putting them in autoclave bag. I've personally had problems when dirt got into my bottles specially when the user before me autoclaved dead chicks or eggs and forgot to completely seal the bag. Just be careful with that also.

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