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Mouse unique genes vs human unique ones


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#1 yhkw

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Posted 27 February 2014 - 09:55 AM

I have culture with mixed human and mouse cells. I need tell which one is which. Does anyone know the unique gene list for mouse and human, so that I can design PCR primers to differentiate them?

Thank you in advance!



#2 bob1

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Posted 27 February 2014 - 12:33 PM

I'm not sure, but I think that you will find homologues for more than 99% of mouse genes in humans - you will probably have to find ones with significant length or sequence differences, such that they can be distinguished by PCR or sequencing.



#3 perneseblue

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Posted 27 February 2014 - 08:05 PM

Yes, you can.

 

B transposon repeats are unique to mouse cells and not found in human cells.

 

I am not in the lab at the moment. I'll get you the mouse primer sequence my lab uses once I'm back at work.


May your PCR products be long, your protocols short and your boss on holiday

#4 yhkw

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Posted 28 February 2014 - 08:30 AM

Thanks for your inputs.

I found two genes from this article "Of Mice and Not Men: Differences between Mouse and Human Immunology"

Glycam1 (mouse present, human absent)

GNLY (mouse absent, human present)






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