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16S / NGS and Bacillus

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#1 firefly2280



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Posted 27 February 2014 - 05:23 AM

Hi all


Not sure if this is the right forum area but I'll give it a try. I've been doing some community analysis work with V5-V7 regions of the 16S gene from the indoor environment. When I look at contact agar plates taken from the same location, the plates have a lot of Bacillus, however, very little if any of my amplicons from my 16S NGS library were identified as Bacillus


Does anyone know the reason for this please? 




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