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can i use old blood


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4 replies to this topic

#1 semsem shaltout

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Posted 26 February 2014 - 10:24 AM

dear:all

can i use blood after 24hr.preservation in refrigerator for intracellular and surface staining in flow?

 

if i used frozen BPMCS in intracellular staining what is the precaution specific for flowcytometry after thawing?and should use immediatly after thawing ?can i transport cells in ice books for 15 min.?

 

kind regard

asmaa



#2 Inbox

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Posted 26 February 2014 - 10:57 AM

Not sure. But what if some of cells disrupt? it would produce background noise!!!



#3 semsem shaltout

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Posted 26 February 2014 - 11:11 AM

Not sure. But what if some of cells disrupt? it would produce background noise!!!

is this for both questions or the 1st only?



#4 Tabaluga

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Posted 26 February 2014 - 03:44 PM

Generally cells should be thawed quickly. As to your last question, transporting on ice should not be a problem.

I just typed     pbmc flow cytometry thawing     into google and got some interesting results and protocols, such as:

 

 

https://www.research...en_thawed_PBMCs

http://www.altaweb.i...PBMC_triage.pdf

http://www.biomedcen...172-6-17-s1.pdf

 

and a lot more...Maybe you shoud just read some of them. Hope it helps :)


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#5 Inbox

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Posted 27 February 2014 - 11:14 AM

1st. Disrupted cells could come outside staining medium (Sheath fluid or whatever).

See above links by Tabaluga. Need to follows correct freeze - thaw protocol. 

 

These protocols are for PBMC. In first question when you mention blood, you mean PBMC right?


Edited by Inbox, 27 February 2014 - 08:47 PM.





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