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secondary antibody creation


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#1 luckyjade

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Posted 25 February 2014 - 10:28 PM

Hello,

 

iif for instance a primary antibody is generated from a rabbit, then the secondary antibody needs to come from a different animal species.  what is the reason why this needs to be done? Is it so that there aren't any rabbit antibody bands generated?



#2 mdfenko

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Posted 26 February 2014 - 05:33 AM

because it is difficult to get an organism to make antibodies against itself (autoimmune diseases notwithstanding).


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