Dear all,
I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.
Thank you.
Cheers,
dee
Best Answer Trof, 26 February 2014 - 10:50 AM
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
Go to the full postPosted 25 February 2014 - 07:05 PM
Dear all,
I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.
Thank you.
Cheers,
dee
Posted 26 February 2014 - 10:50 AM Best Answer
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 26 February 2014 - 08:04 PM
Thank you Trof.
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