Dear all,
I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.
Thank you.
Cheers,
dee
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Different annealing temp between HKG and target gene
Started by detriar, Feb 25 2014 07:05 PM
annealing rt-pcr real-time housekeeping gene reference gene
Best Answer Trof, 26 February 2014 - 10:50 AM
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
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2 replies to this topic
#2
Trof
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Posted 26 February 2014 - 10:50 AM Best Answer
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
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#3
detriar
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Posted 26 February 2014 - 08:04 PM
Thank you Trof.
Also tagged with one or more of these keywords: annealing, rt-pcr, real-time, housekeeping gene, reference gene
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