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Different annealing temp between HKG and target gene

annealing rt-pcr real-time housekeeping gene reference gene

Best Answer Trof, 26 February 2014 - 10:50 AM

Yes.

As long as you run all the standards for each gene on the same plate/run/temperature.

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#1 detriar

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Posted 25 February 2014 - 07:05 PM

Dear all,

 

I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.

Thank you.

 

Cheers,

dee



#2 Trof

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Posted 26 February 2014 - 10:50 AM   Best Answer

Yes.

As long as you run all the standards for each gene on the same plate/run/temperature.


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#3 detriar

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Posted 26 February 2014 - 08:04 PM

Thank you Trof.







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