Dear all,
I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.
Thank you.
Cheers,
dee
Submit your paper to J Biol Methods today!
Different annealing temp between HKG and target gene
Started by detriar, Feb 25 2014 07:05 PM
annealing rt-pcr real-time housekeeping gene reference gene
Best Answer Trof, 26 February 2014 - 10:50 AM
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
Go to the full post
2 replies to this topic
#2
Trof
-
- Global Moderators
-
- 1,344 posts
Brain on a stick
134
Excellent
Excellent
Posted 26 February 2014 - 10:50 AM Best Answer
Yes.
As long as you run all the standards for each gene on the same plate/run/temperature.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
#3
detriar
-
- Active Members
-
- 35 posts
Enthusiast
2
Neutral
Neutral
Posted 26 February 2014 - 08:04 PM
Thank you Trof.
Also tagged with one or more of these keywords: annealing, rt-pcr, real-time, housekeeping gene, reference gene
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Gene expression in colonic tissues using qRT-PCRStarted by Shima Bibi, 20 Sep 2016  RNA, Housekeeping gene and 1 more...
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
reliable homemade mastermix with SybrgreenI for rt-pcrStarted by lorenzo, 23 May 2016 mastermix, RT-PCR, Sybr-green
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
testing primers efficiency for rt-PCRStarted by madelingirly, 27 Apr 2016 primer, rt-pcr
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Metylation analysis by Epitect II Methyl RT-PCR assayStarted by p.blaze53, 30 Nov 2015 methylation, rt-pcr, epigenetics and 1 more...
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Reference Gene/HKG- every plateStarted by ryc, 02 Mar 2015 qpcr, reference gene
|
|
|
