It is the first time I am using these three set of primers, I would like to optimize the primer concentration beforehand.
My question is what if the optimal concentration between these sets of primers differs, are we allowed to run qPCR with different primer concn?
Primer A--- Forward 150nM, Reverse 300nM
Primer B--- Forward 300nM, Reverse 300nM
Primer C--- Forward 300nM, Reverse 150nM