I am trying to induce beta amyloid aggregation in HEK293 using ab peptide 1-42 (1uM for 7 days, according to "Mechanism of amyloid plaque formation suggests an intracellular basis of Aβ pathogenicity"). Unfortunately, after 4 days all my cells died. We took a look at ab salt composition and realized that 26% is composed by TFA. I would like to know if this salt is toxic for cells or the massive death has another reason.
Thank you in advance!!