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How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as

Cell Culture Tecnhiques

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#1 lkhalil04

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Posted 24 February 2014 - 09:19 AM

Hello,

 

I'm trying to figure out how I can have 2000 cells/ well. 

I thought of doing it like this:

2000/0.2= 10e4 cells/ml

Lets assume I have 100 wells (easier for calculation), then 200ul*100 wells=2e4ul/20ml

So in 20ml, I would need 2e5 cells.

 



#2 tkf

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Posted 24 February 2014 - 11:01 AM

Yes, you will need 20mL of 10^4 cells/mL solution. 200uL of this solution per well will give you 2000 cells/well.






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