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help in long pcr


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#1 Angeline

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Posted 24 February 2014 - 06:22 AM

Are there any tips on amplifying >5kb fragment?



#2 phage434

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Posted 24 February 2014 - 06:38 AM

Use a new enzyme (Phusion or Q5). Use specific primers. You may need additives for high GC DNA (5% of a 1 M betaine solution works well).

Make sure your extension times are long enough (easy with these enzymes).






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