I am working on a BAC plasmid. I have this plasmid in both E.coli DH5a and E.coli K12. What I find strange is , I don't see good expression in E.coli K12. Do anyone of you have encountered the same? If not what would be the possible option to make the plasmid work in E.coli K12. I have tried with different range of inductions. But nothing helped. Both the strains are endonuclease negative.
Thanks. Kindly help