How to identify plasmid
Posted 02 June 2004 - 06:40 AM
Now I have constructed one expression plasmid but it is one kind of low
copies. If using PCR amplification, only weak bands can be detected,
but if I use restriction enzyme to cut the plasmid, I can not see any target
bands to be cut after running agrose glue.
Why? And I wander if there are other better methods to identify my
plasmid, I hope someone can help me solve this problem.
Thank a lot !
Posted 02 June 2004 - 11:09 AM
Edited by phdconsult, 02 June 2004 - 11:15 AM.
Posted 03 June 2004 - 03:40 AM
I will have a try at once. Wait for my good news!