I Transformed Gateway plasmid pMDC83 with my 1.5 kb insert to Agrobacterium competent cells, GV3101. The agro colonies are growing after 7-8 days on LB agar plates containing kanamycin 50 microgram/mL and Rifampicin 25 microgram/mL. I have repeated the transformation 4 times. The colony PCR shows the presence of insert. should I proceed for the plant transformation?