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Transformed Agro colonies growing very slowly

molecular Biologe Microbiology Genetics Biochemistry

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#1 awan



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Posted 22 February 2014 - 01:23 AM

Dear Experts

I Transformed Gateway plasmid pMDC83 with my 1.5 kb insert to Agrobacterium competent cells, GV3101. The agro colonies are growing after 7-8 days on LB agar plates containing kanamycin 50 microgram/mL and Rifampicin 25 microgram/mL. I have repeated the transformation 4 times. The colony PCR shows the presence of insert. should I proceed for the plant transformation?

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