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Small yield of RNA after on column DNAs digestion , Why


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#1 Mohamed 1984

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Posted 21 February 2014 - 02:03 PM

Dear All, 

 

I extracted RNA from duck tissue and it was really high quantity 2000 ng/µl  by nanodrop. After that I repeated the extraction with on coulmn DNAse digestion and the quantity dramatically dropped down to 120 ng/µl 

 

What is cause, is the Nanodrop measure the RNA and the DNA together ? 



#2 phage434

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Posted 21 February 2014 - 04:50 PM

Yes, it fails to distinguish them (except for the difference in A280/A260 which is minor, and can't be trusted). You may have introduced RNAses in your DNAse treatment.



#3 Mohamed 1984

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Posted 23 February 2014 - 01:10 PM

May be, but if we assume a nice condition, is this is normal that your RNA quantity reduced after DNAse digestion?



#4 phage434

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Posted 23 February 2014 - 03:08 PM

No. But you can lose RNA in the cleanup afterwards.






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