Odd question maybe but I have some extracted genomic DNA and need to pool and concentrate the samples. I have already submitted my PhD thesis so there isn't any money left to buy a gDNA clean up kit... So I was wondering if pooling them and then using the DNA extraction kit and columns I have then eluting in a smaller volume might work?
I'm only worried as you have to add lysis buffer to get the DNA to bind and wondered what that might do to the DNA.
I checked and cant use PCR clean up kits etc (not that I have one) as they are optimised for smaller fragments. and I dont have one. Also, ethanol precipitation might work but Im not sure I have the reagents and the DNA in my samples is REALLY low.
I would appreciate your thoughts please