I have a question about the use of lipofectamine 2000 for transfection of 293t cells. i am performing the transfection to confirm for functionality of certain plasmids. i am wondering if the amount of lipo 2000 that i put into a well makes a significant difference? the protocol recommends 1 uL-2.5 uL of lipo for each well and i only used 1 uL. will this make a significant difference? what effects are there for using a smaller or greater amount of lipo?