Sir, I kept the culturing temperatures at around 37 degrees as it gets really hot at the place where the mines are situated. The temp touch 48 degress in peak summers. Sir, since scheelite is not soluble, then do you think I would have isolated any relevant bugs? Because I used sodium tungstate to screen them out.
As for the addition of broth directly into the soil, could I dilute it and then release it? Also Sir, I intend to autoclave the soil before adding in the broth, do you think it will reduce the chances of eutrophication?
Sir for bioremediation, I am going to use an uninoculated W concentration as reference. Sir, before I proceed for the bioremediation experiment, I will inoculate a dried biomass of my bugs in the M9 broth, (without a C source) as you said, with a pre-decided conc of tungsten at which my bugs would remain viable and grow (will do MIC to determine the right W concentration). Then with the help of atomic absorption spectroscopy I will determine which inoculated broths show a decrease in W conc after 24 and 72 hours.
Sir, here's a bit of a problem that I would like you to help me out with. How should I separate out my bug's biomass from the inoculated M9 broth cultures without bursting them? I wish to collect only the broth without the cells after 24 and 72 hours of inoculation to check the W conc in it. Should I simple centrifuge them at low rpms, like at around 2000 rpm? So that my cells do not burst and release whatever W they might have accumulated back into the broth that will be collected for AAS analysis?
I will make sure I don't use a broth that may cause precipitation of W.
No Sir, I do not plan on removing the bugs from the soil after treatment. This is actually only a preliminary analysis that I am doing.
Sir could you please provide me with the brewery yeast as biosorbent paper? Also the following paper.
Enterococcus faecalis strain LZ-11 isolated from Lanzhou reach of the Yellow River is able to resist and absorb Cadmium. by Wu G1, Sun M, Liu P, Zhang X, Yu Z, Zheng Z, Chen Y, Li X.
P.S. By the way Sir, I am really very grateful to you for all the help that you are providing me with. I would have hit a roadblock, had it not been for this guidance.