Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

co-transfection


  • Please log in to reply
2 replies to this topic

#1 laur

laur

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 17 February 2014 - 01:58 AM

Hi everyone,

 

I was wondering whether there are any pitfalls in the co-transfection of DNA plasmids using the same vector backbone into mammalian cells. I have a vague memory that in some circumstance that this is problematic, I may have been thinking of bacterial cells and/or co transformation......? A reminder of these details would also be great.

 

Thanks for your thoughts,

 

Laur

 

Please don't double post - bob.


Edited by bob1, 17 February 2014 - 11:29 AM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,833 posts
415
Excellent

Posted 17 February 2014 - 11:31 AM

Not that I am aware of.  For bacterial cells it is problematic if you want to add more than one plasmid with the same resistance marker (e.g. tetr) as it is difficult to tell if all plasmids are present in any one colony.



#3 laur

laur

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 17 February 2014 - 05:22 PM

Thank you.

 

Sorry about the double post, I did not realise I had done that.

 

 

Cheers!






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.