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help on an experiment


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#1 molbio1234

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Posted 16 February 2014 - 10:13 AM

Can one sequence multiple vectors in one pool using NGS without any problems and differentiate between the vector sequences if the vectors are unbarcoded? 

 

If I have 3 different siRNA vectors each with an insert, and transduce them into cells, and extract the DNA from the cells and want to do sequencing on the DNA, how to detect from which vector the insert came from?  Is it possible? 



#2 Inbox

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Posted 02 March 2014 - 02:12 AM

are vectors u r suing r same or different?






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