What do sick human embryonic kidney (293t) cells look like? What are they normally supposed to look like?
What do sick 293t cells look like?
Posted 15 February 2014 - 10:11 PM
How are they sick - old? infected (mycoplasma, other bacteria, fungi)?, drug treatment?, over growth?... etc?
Have a look for pictures on the ATCC website.
Posted 16 February 2014 - 10:44 PM
The ATCC has pictures of healthy cells for most cell lines. I would start there for comparison.
If the cells are contaminated it would be best to throw them out and get up a new tube. Trying to decontaminate such a common cell line is a waste of time and is quite difficult to do properly without altering the cells themselves.