Could anyone give me a help. I sent homognized sample from ducks to Bioanalyzer unite to make aquality assurance test prior to small RNA seq. Some of the sample came without RIN. Some of them show a smeared Band. Others with a RIN of 2.3 Actually I sent 300ng/µl of RNA and the person in the unite diluted the sample 1:10 and he used a Pico chip for the analysis. What might be the cause of this ? Do you think that this results can be suitable for small RNA seq knowing that these small RNAs are more stable.
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