Hallo all, I am using the gateway system however I have a problem with using both an N and C tag.
would it be correct to simple use the reading frame A (my vector ends with a triplet (3 bp) right before where I want to insert the gatewaysystem) and just make sure that at the other side I make sure I have 1 bp extra and than 3 bp?
Meaning: vector GCG - gatewaycassette ending with GTG AT - 1bp - rest of vector ?
Seems to make sense based on the gatewayinformation from invitrogen.
Edited by lyok, 14 February 2014 - 01:53 PM.